Evaluation of Adhesive Characteristics of L. plantarum and L. reuteri Isolated from Weaned Piglets.

Matteo Dell’Anno,Serena Reggi,Tamil Selvi Sundaram,Luciana Rossi,Mariagrazia Cavalleri,Elisabetta Onelli,Alessandra Moscatelli,Carlotta Giromini,Simona Coranelli,Antonella Baldi,Raffaella Rebucci,Ambra Spalletta

doi:10.3390/microorganisms9081587

Matteo Dell’Anno, Serena Reggi + Show 10 more

Open Access

https://doi.org/10.3390/microorganisms9081587

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Journal: Microorganisms	Publication Date: Jul 26, 2021
Citations: 7	License type: CC BY 4.0

Affiliation: University of Milan

Abstract

Limosilactobacillus reuteri and Lactiplantibacillus plantarum strains, previously isolated from weaned piglets, were considered for the evaluation of their adhesive characteristics. Lactobacilli were treated with LiCl in order to remove the surface protein layer, and probiotic activity was compared with those of untreated strains. The autoaggregation, co-aggregation to E. coli F18+, and adhesive abilities of LiCl-treated Limosilactobacillus reuteri and Lactiplantibacillus plantarum were significantly inhibited (p < 0.05) compared with the respective untreated strain. The hydrophobic and basic phenotypes were observed due to the strong affinity to chloroform and low adherence to ethyl acetate. In particular, L. plantarum showed higher hydrophobicity compared to L. reuteri, which may reflect their different colonizing ability. After treatment with LiCl to remove surface proteins, the adherence capabilities of L. reuteri and L. casei on IPEC-J2 cells decreased significantly (p < 0.001) and L. reuteri adhered more frequently. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) showed that both L. reuteri and L. plantarum had several bands ranging from 20 to 100 kDa. Two-dimensional gel electrophoresis showed an acidic profile of the surface-layer polypeptides for both bacterial strains, and more studies are needed to characterize their profile and functions. The results confirm the pivotal role of surface proteins in the probiotic potential of L. reuteri and L. plantarum.

Highlights

Lactobacilli are part of the common flora in the porcine digestive tract [1]
In our attempt to study the adhesive characteristics in the probiotic activity of L. reuteri and L. plantarum, we investigated Limosilactobacillus reuteri and Lactiplantibacillus plantarum autoaggregation, co-aggregation capacity to Escherichia coli
The autoaggregation and co-aggregation ability to E. coli F18+ of L. reuteri decreased significantly after LiCl treatment compared to the untreated strain (p < 0.05), while L. plantarum showed that LiCl treatment did not impair its co-aggregation ability to E. coli (Figure 3)

Summary

Introduction

Lactobacilli are part of the common flora in the porcine digestive tract [1]. Showed that the Lactobacillus genus accounts for approximately 15% of 16S rRNA in intestinal pig samples, regardless of age. While the swine faecal microbiota changed significantly across growth stages, the populations of lactobacilli remain almost stable. Several studies have shown the positive impact of Limosilactobacillus reuteri and Lactiplantibacillus plantarum, previously known as the Lactobacillus genus, on piglet’s performance improvement, diarrhoea prevention, stress alleviation, immunity, microbiota modulation, and carcass quality [3,4], they are not listed in the European register of additives for pigs. L. plantarum can be found in the European Register of Feed Additives according to European Regulation [5], under

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