Evaluation of Addition The Activated Charcoals and pH Adjustment in The Treatment of Lignocellulosic Hydrolisates for Xylitol Production

Abstract

D-Xylitol is a five-carbon sugar alcohol, which can be obtaines from xylose of agriculutural wastes with microbial fermentation. The inhibitory chemical complexes (ICC) were founded after biomass pretreatment step. The ICC in acid hydrolysate can inhibit microbial metabolism in the medium. Several methods for detoxification; adsorption by activated charcoal, by ion exchange resin or by using enzymes. This study aims to evaluate the effect of using charcoal in xylitol fermentation by using lignocellulosic hydrolisate. In this work, detoxification process by added 1% activated charcoal to reduce the inhibitors in corn cobs hydrolysate and sugarcane trash hydrolysate. The biomass hydrolysate was fermented by Meyerozyma caribbica Y67 to produce xylitol. It is expected that detoxification can reduce concentration of inhibitors, so that microbial growth is not inhibited and more product. The results showed that M. caribbica Y67 had not significant different between pure hydrolysate and detoxified hydrolysate with activated charcoal. We suggested that M. caribbica Y67 tolerance to ICC and potential for xylitol fermentation from lignocellulosic biomasses.