EVALUATION OF ACUTE DERMAL IRRITATION, SENSITIZATION, AND IN-VITRO ANTI-INFLAMMATORY ACTIVITY OF CANNABIS-BASED PAIN-RELIEVING TOPICAL OIL – CANNAQUICTM

Abstract

Cannabis is known and considered a sacred plant because of its analgesic, anti-inflammatory, and stimulating properties. Other herbs like Shallaki, Bala, Nirgundi, Kantakari, and Kupilu are also known as medicinal herbs and have tremendous pain-relieving properties. Objectives: A formulation with an infusion of cannabis leaves extract and other herbs in sesame oil can be a good therapy for relieving different types of pain when applied topically. An evaluation study was conducted in animals for the evaluation of dermal irritation, sensitization, and anti-inflammatory properties. Methods: This study was designed to evaluate acute dermal irritation/corrosion, sensitization on skin and anti-inflammatory activity of CannaquicTM according to OECD guidelines. No erythe-ma, edema, or any skin lesion was observed after application. Results & Conclusion: The results revealed no irritation/corrosion from CannaquicTM. In-vitro Anti-Inflammatory activity against LPS-induced toxicity in Mouse Macrophage (RAW 264.7) cell line exhibits a significant percentage reduction in IL-6, Nitric Oxide (NO), and TNF-alpha in Mouse Macrophage (RAW 264.7) cell-line against LPS-induced inflammation.