EVALUATION OF ACUTE AND SUBCHRONIC TOXICITY OF PRAVAD FEED ADDITIVE

Abstract

The article presents results of studies of a new highly effective feed additive "Pravad" based on probiotics, adaptogenes, vitamins and amino acids, which has shown itself to advantage in fish farming. Acute toxicity of Pravad feed additive was studied on laboratory animals (rats) and the hazard class was established when it was orally administered. Subchronic toxicity of the feed additive was studied, its results allowto identify the presence or absence of a combination of functional and/or morphophysiological disorders of organs and systems in case of its application. The components that make up the new feed additive are, according to State Standard GOST 12.1.007, low-toxic substances of the 4th hazard class. The obtained results on the study of acute and subchronic toxicity of Pravad feed additive showit has no toxic effect. Long-term observations of laboratory animals on which Pravad feed additive was tested did not reveal any signs of somatic distress. The results of assessment of biochemical parameters and leukocyte formula did not reveal significant differences among parametres of the experimental and control groups. This indicates that introduction of Pravad feed additive into the ration does not have a negative impact on homeostasis of animals. Intragastric administration of Pravad feed additive did not cause significant changes in the mass coefficients of kidneys, lungs, spleen, heart and liver compared to control animals. Pathological and anatomical autopsy of the animals of the experimental group did not reveal any deviations of internal organs in comparison with the animals of the control group.