Abstract
High-performance liquid chromatography with a UV-VIS linear diode array (LDA) detector was used for the analysis of dyes. The development of a computer program enabled absorbance ratios to be calculated from the analytical data. An evaluation of these ratios for identifying the dye components has been made. Precisions of approximately 2% relative standard deviation can be achieved for absorbance ratios obtained from single and multi-component dye samples within the wavelength range of 210–600 nm provided that the absorbance values are greater than 1.0 mAU. A comparison of methods for generating absorbance ratios from dye samples over a range of 335 pg to 530 ng indicate that ratios derived from peak height measurements are more reproducible than those obtained from peak area measurements. The application of absorbance ratioing and the potential of the technique using the LDA detector are also presented.
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