Evaluation of Abelmoschus Esculentus Seed for Antioxidant and Phytochemical Analysis Using In vitro Assays

Abstract

The aim of this study was to assess the in vitro potential of methanolic seed extract of Abelmoschus esculentus as a natural antioxidant. The DPPH activity of the Ethyl acetate soluble fraction (10, 20, 40, 80, and <TEX>$160{\mu}g/mL$</TEX>) was increased in a dose dependent manner, which was found in the range of 18.97-90.47% as compared to ascorbic acid 26.44-93.71%. The <TEX>$IC_{50}$</TEX> values of Ethyl acetate soluble fraction (EAES) and ascorbicacid in DPPH radical scavenging assay were obtained to be 28.12 and <TEX>$18.43{\mu}g/mL$</TEX>, respectively. Measurement of polyphenol content of the EAES of A. esculentus seed was achieved using Folin-Ciocalteau reagent containing 53.80 mg/g of total phenolic content, which was found signicantly higher when compared to reference standard gallic acid. Similarly total flavonoids and proabthocyanidis of EAES and chloroform soluble fraction (CAES) were found significantly 147 mg/g and 14.24 mg/g respectively when both compared to reference standard quercetin. EAES exhibited high significant lipid peroxidation inhibition effects in a dose-dependent manner, with <TEX>$IC_{50}$</TEX> values of <TEX>$38.08{\mu}g/mL$</TEX>, whereas, standard quercetin, with <TEX>$IC_{50}$</TEX> value of <TEX>$36.67{\mu}g/mL$</TEX>. All extract/fractions showed dose dependent reducing power ability and these differences were statistically significant (p<0.001). The results obtained in this study clearly indicate that A. esculentus seed has a signicant potential to use as a natural antioxidant agent.