Evaluation of a testicular sperm head counting technique using rats exposed to dimethoxyethyl phthalate (DMEP), glycerol alpha-monochlorohydrin (GMCH), epichlorohydrin (ECH), formaldehyde (FA), or methyl methanesulphonate (MMS).

Abstract

Single dosages of DMEP (1,000-2,000 mg/kg), GMCH (50 mg/kg), ECH (25 and 50 mg/kg), FA (100 and 200 mg/kg), and MMS (100-400 mg/kg) were administered orally to 10 week old male Wistar rats. The rats were necropsied on the 11th day following dosing. The testes were weighed, homogenised and sonicated; numbers of sperm heads (total and abnormal) were counted and percentage sperm head abnormalities were calculated. Testes weights were significantly reduced only in rats exposed to 1,500 and 2,000 mg DMEP/kg. Compared with controls, there were significant increases in the incidence of abnormal sperm at all dose levels of MMS and the higher dose levels of DMEP (1,500 and 2,000 mg/kg), ECH and FA. No toxicologically significant effects upon total sperm counts were seen following the oral administration of any of the five chemicals tested. However, an additional group of rats given 100 mg MMS/kg intraperitoneally (i.p.) showed significant reductions in testes weight and total sperm head counts compared with control animals. It is concluded that this testicular sperm head counting technique is a useful tool in the detection of selective adverse effects of chemicals upon testicular sperm but requires further evaluation.