Abstract
The properties of four ruthenium complexes and their efficiency as probes for chemiluminescent (CL) detection were evaluated, and a protocol for CL detection of a protein labeled with a tertiary amine was proposed. Of the four ruthenium complexes studied, Ru(bpy) 3 3+ proved to exhibit the characteristics necessary for optimum CL detection. Two biotin derivatives were synthesized containing a tertiary amine functionality and then incubated with the protein, avidin. The addition of the tertiary amine moiety to biotin made the derivative more sensitive to Ru(bpy) 3 3+-based CL. Quantitative data were obtained as a model of a labeling protocol by varying the number of biotins per avidin. Limit of detection (LOD) for either derivative were determined to be ∼50 nM, for the lowest concentration studied. The relative luminescence response increased with increasing number of tertiary amine labels per molecule of avidin. The results showed the potential of this approach for the detection of proteins using this tertiary amine labeling methodology.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
