Abstract
Seventy food samples (23 samples of raw milk, 24 samples of minced meat and 23 samples of unsalted butter) were examined using the direct epifluorescent filter technique (DEFT). For each sample, micro-organisms captured on duplicate filters were counted both by eye and with the aid of Bio-Foss 40-10 image analyser. Each count was performed by two operators. The correlation coefficients for the image analyser counts against the manual DEFT count for the 3 products ranged from 0.94 to 0.98. An analysis of variance indicated that both counting methods were equivalent in their repeatability. Operator experience and the quality of the microscopic image were important factors in determining the accuracy of the automatic count.
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