Abstract
A scattering microscope was developed to investigate single cells and biological microstructures by light scattering measurements. The spectrally resolved part of the setup and its validation are shown in detail. The analysis of light scattered by homogenous polystyrene spheres allows the determination of their diameters using Mie theory. The diameters of 150 single polystyrene spheres were determined by the spectrally resolved scattering microscope. In comparison, the same polystyrene suspension stock was investigated by a collimated transmission setup. Mean diameters and standard deviations of the size distribution were evaluated by both methods with a statistical error of less than 1nm. The systematic errors of both devices are in agreement within the measurement accuracy.
Highlights
Scattered light is often an annoying phenomenon in nature, whether it is fog outdoors or unwanted stray light inside the optics laboratory
Many conclusions on the structure, the size or the optical properties of a medium can be drawn by the analysis of scattered light
The results are based on two different methods, particles in suspension measured by the collimated transmission and single particles measured by the scattering microscope
Summary
Scattered light is often an annoying phenomenon in nature, whether it is fog outdoors or unwanted stray light inside the optics laboratory. The scattering patterns can be observed spectrally resolved [1, 2], angular resolved [3, 4], polarization dependent [5], time resolved [6, 7] or spatially resolved [8, 9]. In this contribution a scattering microscope is presented that combines spectroscopic and angular resolved measurements, similar to the setup presented by Cottrell et al [17]. The presented microscope works on the basis of a reflected darkfield illumination. This is advantageous for the observation of thicker or strongly absorbing samples. In case of Mie scattering, the spectral and angular patterns of the backward scattered light contain more information. In comparison to Cottrell’s setup [17] or the 4D-ELF setup published by Roy et al [20], the range of detected scattering angles is enlarged in our setup (from 93◦ to 157◦)
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