Abstract
Chikungunya virus (CHIKV) is becoming an increasing global health issue which has spread across the globe and as far north as southern Europe. There is currently no vaccine or anti-viral treatment available. Although there has been a recent increase in CHIKV research, many of these in vitro studies have used a wide range of cell lines which are not physiologically relevant to CHIKV infection in vivo. In this study, we aimed to evaluate a panel of cell lines to identify a subset that would be both representative of the infectious cycle of CHIKV in vivo, and amenable to in vitro applications such as transfection, luciferase assays, immunofluorescence, western blotting and virus infection. Based on these parameters we selected four mammalian and two mosquito cell lines, and further characterised these as potential tools in CHIKV research.
Highlights
Chikungunya virus (CHIKV) is an enveloped, positive sense, single stranded RNA virus[1]
We propose that four mammalian cell lines, Huh[7], C2C12, SVG-A and dermal fibroblasts and two mosquito cell lines, U4.4 and C6/36 provide good models for in vitro CHIKV research
We initially evaluated a panel of mammalian cell lines for their ability to support CHIKV genome replication
Summary
Chikungunya virus (CHIKV) is an enveloped, positive sense, single stranded RNA virus[1]. Recently mutations in the E1 and E2 glycoproteins (E1-A226V and E2-I211T respectively) have enabled one genotype of the virus (ECSA: East/Central/South African) to more efficiently infect Aedes albopictus due to enhanced infectivity of the mid-gut[4]. This mosquito can tolerate more moderate climates[5]. Cell line Huh[745] C2C1246 SVG-A47 Dermal fibroblasts[42] (see methods) HepG248 RD49 BHK-2128 A54950 HeLa51 Vero E652 A2053 Aag[254] U4.454 C6/3654 Aedes albopictus this we have evaluated and compared cell lines from a range of origins for their use with CHIKV – using both sub-genomic replicon (SGR) systems and infectious virus
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