Abstract
Background. The Allergy Lateral Flow Assay (ALFA) is a novel rapid assay for the detection of sIgE to allergens. The objective of this study is the evaluation of ALFA for the detection of sIgE to bee venom (BV) and wasp venom (WV) in insect venom allergic patients. Methods. Specific IgE to BV and WV was analyzed by ALFA, ALLERG-O-LIQ, and ImmunoCAP in 80 insect venom allergic patients and 60 control sera. Sensitivity and specificity of ALFA and correlation of ALFA and ImmunoCAP results were calculated. Results. The sensitivity/specificity of ALFA to the diagnosis was 100%/83% for BV and 82%/97% for WV. For insect venom allergic patients, the Spearman correlation coefficient for ALFA versus ImmunoCAP was 0.79 for BV and 0.80 for WV. However, significant differences in the negative control groups were observed. Conclusion. ALFA represents a simple, robust, and reliable tool for the rapid detection of sIgE to insect venoms.
Highlights
Reliable diagnosis of insect venom allergy is based on the combination of patient’s history, skin testing (SPT) and laboratory tests for the detection of specific IgE [1,2,3,4].The methodology for the measurement of sIgE has evolved during the recent years [5, 6]
The objective of this study is the evaluation of Allergy Lateral Flow Assay (ALFA) for the detection of sIgE to bee venom (BV) and wasp venom (WV) in insect venom allergic patients
Specific IgE to BV and WV was analyzed by ALFA, ALLERG-O-LIQ, and ImmunoCAP in 80 insect venom allergic patients and 60 control sera
Summary
Reliable diagnosis of insect venom allergy is based on the combination of patient’s history, skin testing (SPT) and laboratory tests for the detection of specific IgE (sIgE) [1,2,3,4].The methodology for the measurement of sIgE has evolved during the recent years [5, 6]. Reliable diagnosis of insect venom allergy is based on the combination of patient’s history, skin testing (SPT) and laboratory tests for the detection of specific IgE (sIgE) [1,2,3,4]. The Allergy Lateral Flow Assay (ALFA) is a novel rapid assay for the detection of sIgE to allergens. The objective of this study is the evaluation of ALFA for the detection of sIgE to bee venom (BV) and wasp venom (WV) in insect venom allergic patients. Specific IgE to BV and WV was analyzed by ALFA, ALLERG-O-LIQ, and ImmunoCAP in 80 insect venom allergic patients and 60 control sera. For insect venom allergic patients, the Spearman correlation coefficient for ALFA versus ImmunoCAP was 0.79 for BV and 0.80 for WV. ALFA represents a simple, robust, and reliable tool for the rapid detection of sIgE to insect venoms
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