Abstract
Y. enterocolitica and Y. pseudotuberculosis are important food borne pathogens. However, the presence of competitive microbiota makes the isolation of Y. enterocolitica and Y. pseudotuberculosis from naturally contaminated foods difficult. We attempted to evaluate the performance of a modified Cefsulodin-Irgasan-Novobiocin (CIN) agar in the differentiation of Y. enterocolitica from non-Yersinia species, particularly the natural intestinal microbiota. The modified CIN enabled the growth of Y. enterocolitica colonies with the same efficiency as CIN and Luria-Bertani agar. The detection limits of the modified CIN for Y. enterocolitica in culture medium (10 cfu/ml) and in artificially contaminated pork (104 cfu/ml) were also comparable to those of CIN. However, the modified CIN provided a better discrimination of Yersinia colonies from other bacteria exhibiting Yersinia-like colonies on CIN (H2S-producing Citrobacter freundii, C. braakii, Enterobacter cloacae, Aeromonas hydrophila, Providencia rettgeri, and Morganella morganii). The modified CIN exhibited a higher recovery rate of Y. enterocolitica from artificially prepared bacterial cultures and naturally contaminated samples compared with CIN. Our results thus demonstrated that the use of modified CIN may be a valuable means to increase the recovery rate of food borne Yersinia from natural samples, which are usually contaminated by multiple types of bacteria.
Highlights
IntroductionAmong many species of Yersinia, Y. enterocolitica and Y. pseudotuberculosis are food borne pathogens and are a frequent cause of human yersiniosis [1]
Yersinia are Gram-negative bacteria belonging to the family Enterobactericeae
Both CIN and modified CIN allowed the growth of all Yersinia strains tested with the exception of the non-pathogenic strain IP102 (Y. enterocolitica bioserotype 1A/O:6,30), which was inhibited on both media
Summary
Among many species of Yersinia, Y. enterocolitica and Y. pseudotuberculosis are food borne pathogens and are a frequent cause of human yersiniosis [1]. Y. enterocolitica is one of the ten most important food borne enteric pathogens in Europe and the United States [1,2]. Y. enterocolitica infections usually results from the consumption of contaminated food or water, and the disease tends to affect young children [1,3]. The symptoms of yersiniosis include acute gastroenteritis with fever, bloody diarrhoea, and pseudo-appendicitis, which frequently leads to unnecessary laparotomy [4]. Y. pseudotuberculosis infections are less frequent than Y. enterocolitica infections. They are more common in adults and typically cause lower right abdominal pain that resembles appendicitis and, less frequently, diarrhea [1]
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