Abstract
Cases of melioidosis and glanders are rare in the United States, but the etiologic agents of each disease (Burkholderia pseudomallei and Burkholderia mallei, respectively) are classified as Tier 1 select agents because of concerns about their potential use as bioterrorism agents. A rapid, highly sensitive, and portable assay for clinical laboratories and field use is required. Our laboratory has further evaluated a latex agglutination assay for its ability to identify B. pseudomallei and B. mallei isolates. This assay uses a monoclonal antibody that specifically recognizes the capsular polysaccharide produced by B. pseudomallei and B. mallei, but is absent in closely related Burkholderia species. A total of 110 B. pseudomallei and B. mallei were tested, and 36 closely related Burkholderia species. The latex agglutination assay was positive for 109 of 110 (99.1% sensitivity) B. pseudomallei and B. mallei isolates tested.
Highlights
The Gram-negative bacteria Burkholderia pseudomallei and Burkholderia mallei are the etiologic agents of melioidosis and glanders, respectively
This assay uses a monoclonal antibody that recognizes the capsular polysaccharide produced by B. pseudomallei and B. mallei, but is absent in closely related Burkholderia species
B. pseudomallei is commonly dismissed as a culture contaminant, and along with B. mallei may be misidentified by standard identification methods including API 20NE and other automated bacterial identification systems
Summary
CDC2721620 CDC2721628 CDC2721639 CDC0022138 Bp92; CDC2721623 Human 88; PHLS 45 Bp104; CDC2721624 CDC2721635; PHLS 36 Bp73; Ln31348 PHLS 208 CDC2721102; F5013 BpG9709; CDC0032026 PHLS 19; CDC2721625 CDC2721676 Bp2889; SID2889 CDC2721630; 7605 Bp68; CDC2721641 PHLS 17; CDC2721619 PHLS 38 1106a; CDC0022030 Bp53; CDC2721633 Bp24; CDC2721620 BpG9313; CDC0032029 CDC2721162 CDC2721114; G6715 CDC2721626 CDC0032028 CDC721096; 81A442 CDC0032024 Thai NE Human 99 CDC1029240 CDC2721617 Bp14; CDC2721618 BpH1442; CDC0032025 MSHR640;CDC8724880 465a; CDC8724601 MSHR99; CDC8724881 CDC1756207 CDC8724890 #711; CDC2721675 CDC2734678; 620 CDC8724908 CDC8724883 CDC2734694; PM40 PM26; CDC2734683 PHLS 75 CDC8724901 PM115; CDC2734709 CDC2721825 Bp40 CDC8724894 CDC2734661; SA923 PHLS 79 BpH1689; CDC0032024 CDC2721184 CDC2721634 CDC1756205 CDC8724905 CDC0022203 CDC2721637 CDC8724896 CDC8724889 CDC8724898 CDC2721686 CDC8724899 CDC8724882 CDC8724900 CDC8724892 CDC8724893 CDC2721761. France Madagascar Kenya Thailand Australia Thailand Australia Singapore Malaysia Ecuador Georgia India Singapore USA Bangladesh France Fiji Indonesia Singapore Thailand Thailand France USA Australia USA (Ohio) Thailand USA (Ohio) USA (New York) Puerto Rico Thailand USA (Oregon) Australia Philippines USA (Delaware) Australia Australia Australia Australia Australia USA (Washington) Thailand Australia Australia Thailand Thailand Malaysia Australia Thailand Thailand Singapore Australia Thailand Malaysia USA (Florida) Ecuador Thailand Australia Australia Thailand Pakistan Thailand Australia Australia Australia Thailand Australia Australia Australia Australia Vietnam
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