Abstract
SummaryAn “indirect” fluorescent antibody test for antibodies in cases of amebiasis is described. Antigen consists of washed, intact, formalin-fixed amebae from cultures growing with mixed bacteria. Counter-staining with Evans Blue greatly improves ability to determine end-points in titrations.Antigen from the Huff strain of Entamoeba histolytica, capable of growing at reduced as well as at body temperature, showed far fewer positive reactions than antigen prepared from a virulent, “classic” strain of this species. With the latter antigen, 73.1% of cases with confirmed intestinal amebiasis, and 91.3% of cases with confirmed or unconfirmed extra-intestinal amebiasis were found positive at 1:16 or higher. However, 5 out of 16 individuals without amebic infection were also found positive at low titer. A possible explanation for the false positives and a means for eliminating it are suggested. It is concluded that the test as here presented is technically well-fitted for the experienced laboratory to use, with caution, until more information is accumulated to explain the few anomalous results.
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