Evaluation of a combined fractionation and speciation approach for study of size-based distribution of organotin species on environmental colloids

Abstract

Results relating to the first original application of an analytical approach combining asymmetric flow field-flow fractionation (As-Fl-FFF) with multi-detection and chemical speciation for determination of organotins in a landfill leachate sample are presented. The speciation analysis involved off-line head-space solid-phase microextraction (HS-SPME)-gas chromatography with pulsed-flame photometric detection (GC-PFPD) performed after three consecutive collections of five different fractions of interest from the As-Fl-FFF system and cross-flow part (assumed to be representative of the <10 kDa phase). After 0.45 microm filtration and without preconcentration before fractionation and speciation analysis, limits of detection (LOD) were 4-45 ng (Sn) L(-1) in the sample, with relative standard deviations (RSD) of 3-23%. The As-Fl-FFF fractionation of this sample enables characterization of two distinct populations-organic-rich and inorganic colloids with gyration radius up to 120 nm. Total Sn and mono and dibutyltins (MBT and DBT) appear to be distributed over the whole colloidal phase. Tributyl, monomethyl, monooctyl, and diphenyltins (TBT, MMT, MOcT, and DPhT) were also detected. Quantitative speciation analysis performed on the two colloidal populations and in the <10 kDa phase revealed concentrations from 130 +/- 10 (MMT) to 560 +/- 50 ng (Sn) L(-1) (DPhT).