Evaluation Method for Spore Concentration Uniformity on a Fungal Substrate: Use of a Real-Time Aerosolization Technique

Abstract

Abstract The aerosolization characteristics of the fungal spores are affected by many environmental variables. The uniformity and distribution of spore concentrations on a fungal substrate is an important parameter for the stable and constant generation of fungal bioaerosols over long time periods. However, this traditional method using a light microscope is very slow and inefficient in evaluating the uniformity of spores on fungal substrates. In this study, we propose an evaluation method for spore concentration uniformity on fungal substrates using the real-time aerosolization characteristics of the fungal spores. A fungal bioaerosol generator, developed previously, was used for the steady aerosolization of fungal spores. The multiorifice air jets and the rotating substrate inside the generator were used to scan a fungal culture Petri plate and to aerosolize the fungal spores. Experimental tests on Cladosporium cladosporioides were conducted. The particle size distribution and total spore concentration ...