Evaluation In vitro Antioxidant and Anti-cancer Activity of Rauvolfia tetraphylla L. on Breast Cancer Cell Lines

Abstract

Rauvolfiatetraphylla L. is a significantly endangered woody shrub that belongs to the familyApocynaceaeand it has enormous medicinal properties. Although the plant Rauvolfiatetraphylla is used to treat cancer inthe traditional medicine of some regions, its cytotoxic activity has not been subjected to rigorous investigation.Extracts were obtained from various organic solvents such as Hexane, Chloroform, Ethylacetate andMethanol by the sequential method. The antioxidant activity was accomplished on various crude extractsby 2-diphenyl-1-picrylhydrazyl (DPPH) assay of R. tetraphylla and showed maximum radical scavengingactivity in methanol extract of fruit (IC50 = 60.37 μg/ml) followed by methanol extract of theleaf (IC50 =62.61μg/ml). Screening of in vitrocytotoxicity was performed on all the crude extracts by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay on Breast Cancer cell line (MDAMB231).Among the four-leaf extracts, maximum cytotoxicity activity was obtained by methanol 73.20 μg/mL andthe IC50 value 64.29 μg/ml. Fruit extracts showed maximum cytotoxicity in 65.30 μg/mL methanol andIC50 values were 74.84μg/ml.