Evaluation for Toxicity and Improved Therapeutic Effectiveness of Natural Polymer Co-administered Along with Venocin in Acetic Acid-Induced Colitis Using Rat Model

Abstract

In these present study, two different series of experiments such as evaluation for toxicity and therapeutic of effectiveness of Aesculus hippocastanum co-administered low viscosity of sodium alginate has been carried out by using rat model (Acetic acid-induced ulcerative colitis). Acute and subacute toxicities were performed according to OECD guideline-423 and 407, respectively. In vitro antioxidant study for the combination therapy was studied. Histopathological examinations were carried out to determined organ level toxicity on long-term use of such combination. The results for In vitro antioxidant study suggested the free radical scavenging activity of the combination therapy. There were no behavioral changes or any morbidity and mortality were observed during the oral acute toxicity study food intake, water intake, and body weight variation in all the group of animals were within the similar pattern that means no much changes has been observed. Among the animals between control tests and standard drug-treated groups. The result of histopathological data indicated that some changes were observed for hemoglobin content, red blood cell count, etc., in some test group which is negligible in comparison with control group. The microscopic feature of histopathological study for the different organs such as kidney, liver, heart, etc., indicated that some degeneration and necrosis were observed in all the test groups of animals. These alterations of histopathological changes may be due to the stress, infection, and administration of test compound in empty stomach. Further study is suggested for determination of appropriate dose and ratios (for combination) to reduce the long-term toxicity and to improve the therapeutic effectiveness for the benefit of the entire society.