Abstract
BackgroundAiming to obtain the highest triterpene content in the extraction products, nine bark samples from the forest abundant flora of Apuseni Mountains, Romania were Raman spectroscopically evaluated. Three different natural extracts from Betula pendula Roth birch bark have been obtained and characterized using Fourier transform vibrational spectra.ResultsThis study shows that principal components of the birch tree extract can be rapidly recognized and differentiated based on their vibrational fingerprint band shape and intensity. The vibrational spectroscopy results are supported by the GC-MS data. Based on IR and Raman analysis, one can conclude that all the extracts, independent on the solvent(s) used, revealed dominant betulin species, followed by lupeol.ConclusionsSince Raman measurements could also be performed on fresh plant material, we demonstrated the possibility to apply the present results for the prediction of the highest triterpene content in bark species, for the selection of harvesting time or individual genotypes directly in the field, with appropriate portable Raman equipment.
Highlights
Aiming to obtain the highest triterpene content in the extraction products, nine bark samples from the forest abundant flora of Apuseni Mountains, Romania were Raman spectroscopically evaluated
As the extraction product is consistent with the pentacyclic triterpenes structure as confirmed by the GC-MS, the vibrational FT-infrared spectroscopy (IR) and Raman spectra directly revealed the triterpenes specific content of the raw bark
These raw materials were used to evaluate in detail with vibrational spectroscopy the birch bark composition as a reference point for extracts and to prove the applicability of this technique for such an application
Summary
Aiming to obtain the highest triterpene content in the extraction products, nine bark samples from the forest abundant flora of Apuseni Mountains, Romania were Raman spectroscopically evaluated. Three different natural extracts from Betula pendula Roth birch bark have been obtained and characterized using Fourier transform vibrational spectra. Because of its wide beneficial pharmacological activities, the bark of birch tree has been the subject of respect since ancient times, as well as the more recent subject of exhaustive research and natural extracts industry [1]. Many previously published reports have revealed the occurrence of lupane-based triterpenes across a multitude of plant specie [3]. Plant species from different geographical locations might reveal a variable composition of the extraction product, which is essential to be accurately characterized for biological use, including the content in BA and B
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