Abstract
A total of 90 birds comprising two native breeds viz. Aseel and Kadaknath and one synthetic broiler stock i.e. IBL-80 were utilized to evaluate and compare antibody response to Sheep Red Blood Cells by haemagglutination test at 0, 5 and 10 days post primary inoculation, to study in vivo cell mediated immune response to mitogen Phytohaemagglutinin (PHA-P) and to evaluate immune responsiveness to IBDV vaccine. The presence of natural antibodies against SRBC was evident in all the genetic groups. All groups showed an increase in HA titre upto 10 days post immunization. The HA titre on 10 day was significantly higher in Aseel (1.88±0.10) followed by IBL-80 (1.13±0.05) and Kadaknath (1.09±0.06). However, the differences among Kadaknath and IBL-80 at day 10 PPI were failed to attain statistical significance. The in vivo cell mediated response to mitogen was highest in Aseel (0.68 mm) followed by IBL-80 (0.59 mm) and Kadaknath (0.43 mm).There was significant difference between the layer breeds for response to phytoheamagglutinin but IBL-80 was not significantly differ from both Aseel and Kadaknath. The titre values for IBDV were lowest before immunization and got increased during 7, 14, 21 DPI. At 14 DPI the titre value were significantly different in all the breeds in which Aseel exhibited the highest titre value (2.96±0.04) followed by IBL-80 (2.77±0.09) and Kadaknath (2.64±0.06). It was found that at 21 DPI antibody response was highest in all the breeds, however differences in titre value at 21 DPI in different breeds were not significantly different.
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