Evaluating the stability of individual variation in social and nonsocial behavioural types using prairie voles (Microtus ochrogaster)

Abstract

Prairie voles (Microtus ochrogaster) exhibit remarkable individual variation in social behaviour, suggesting differences in behavioural types. To date, however, there has been little assessment of whether these behavioural types are stable across test sessions, nor to what extent internal states and external contexts (domains) drive individual differences. Here we examined the individual consistency of social (huddling) and non-social (distance moved) behaviour across repeated, long-duration tests, in same-sex cagemate (SS-CM), same-sex stranger (SS-S), opposite-sex stranger (OS-S), and standard partner preference test (PPT) contexts. The SS-CM and SS-S tests were repeated multiple times (SS-CM 1–2; SS-S 1–5) to assess state-dependent variation. A second cohort was used to determine the replicability of findings. Overall, there was a general lack of stability in huddling behavior. It was inconsistent across repeated sessions of the same test type and between types of tests, suggesting a strong contribution of state-dependent variation. Non-social behaviour was more consistent and appeared more domain-dependent and less state-dependent than huddling. Translational and comparative studies of individual variation would likely benefit from testing across multiple contexts and employing repetitive testing paradigms to account for state-dependent variation.