Evaluating the sensitivity of water stressed maize chlorophyll and structure based on UAV derived vegetation indices

Abstract

To further assess the sensitivity of crop chlorophyll and structure based on UAV vegetation indices (VIs) to maize water stress, a study was carried out in a maize field located in Inner Mongolia, China, with various levels of deficit irrigation over the entire 2018 and 2019 growing seasons. Ground measurements of stomatal conductance (Gs), leaf area index and leaf chlorophyll were used as references for maize water status, canopy structure and chlorophyll content, respectively. Four structure VIs and two chlorophyll VIs, and three regression algorithms (multiple linear, random forest and artificial neural networks regression) were adopted. The results showed that canopy structure derived from VIs had a significant correlation (p < 0.001) with Gs with the highest r value of 0.64 (n = 270) in 2018 and 2019. The transformed chlorophyll absorption in reflectance index, chlorophyll VI, could only estimate severe maize water stress with an r value of −0.47 (p < 0.001, n = 270) for the drier 2019. The water stress sensitivity of chlorophyll and structure VIs maybe significantly influenced by different responses of canopy structure and chlorophyll concentration to water stress, and the different spectral resolution of UAV multispectral cameras. Compared to non-linear machine learning regression algorithms, the multiple linear regression was robust enough to relate UAV-based multispectral VIs to Gs with coefficients of determination of 0.48 and 0.45 (n = 270) for 2018 and 2019, respectively. Although stable significant correlations were found between UAV multispectral VIs and Gs, annual changes in these specific expressions were also observed. Overall, our results demonstrated the potential of using structure VIs derived from UAV multispectral images and multiple linear regression approach to estimate maize water status in field scale.