Evaluating the Sensitivity of (miR-378) as a Circulatory Screening Biomarker for Diabetic Cardiomyopathy: Comparative Study

Abstract

Background: Diabetic cardiomyopathy is one of the serious complication of diabetes mellitus, with a silentdevelopment and it is often underestimated, Currently available diagnostic techniques are limited in theirability to identify patients who present with uncommon symptoms or silent myocardiopathy in its earlystages.Aim: To evaluate role of miRNA-378 compared to GLS as a sensitive biomarker for early detection ofsubclinical diabetic cardiomyopathy.Method: This case control study involved 75 DM2 patients from Merjan Medical City in addition to ageand sex-matched 25 apparently healthy subjects . The echocardiographic assessment for GLS was donefor control group (H) and normotensive diabetic patients and these patients were divided into (CD) groupwithout cardiomyopathy, and (M)group with cardiomyopathy diagnosed by negative GLS and positive GLS,respectively. Group (F) include Hypertensive and/or ischemic diabetic patients with overt heart failure .conventional Echocardiography was performed to all patients and control groups, all of them were of normalEF, except group F who have EF less than 40%. Bloodsampling was done for all and miRNA extraction andanalysis done according to the manufacturer recommendations.Results: There is significant elevation in miR-378 Ct value between H in one side & other three diabeticgps. (CD, M, F) as presented in table (3-17) (p< 0.05). There is significant elevation in miR-378 Ct value inM than CD groups. There is significant elevation in Ct value in F than M gp. (p=0.000). In the ROC curve(AUC) was 0.965. The sensitivity and specificity were 0.978 and 0.646, respectively according to the chosencut-off value.Conclusion: This study showed that miRNA-378 could be regarded as a novel sensitive biomarker whichcould play a significant role for early detection of subclinical diabetic cardiomyopathy