Evaluating the Safety of Intravenous Delivery of Autologous Activated Platelet-rich Plasma

Karina Karina,Sulaeha Ad,Krista Ekaputri,Sarah Listyo Astuti,Imam Rosadi,Alfida Zakiyah,Sristin Indah Prestiani,Grady Krisandi,Meyla Shinta Mutiara,Difky Ernanda,Noor Aini,Azza Maryam,Tias Widyastuti,Irsyah Afini,Indah Mustika Donna,Yuliardy Limengka,Iis Rosliana,Siti Sobariah,Wismo Reja Subroto,Ratna Herawati Purwoko ,Nelfidayani ,Tommy P Sibuea ,Jusryanti ,S Agustini ,Habibi Habibi ,Anastasia Maria Loho ,Johannes Albert Biben

doi:10.17532/jhsci.2021.1276

Abstract

Introduction: Autologous platelet-rich plasma (PRP) has been a growing trend in the field of medicine due to its broad range of application and is considered safe from bloodborne diseases. Furthermore, various studies have tried to optimize the use of autologous PRP through various preparation protocols, including PRP activation. However, most of the studies available have not evaluated the safety for intravenous delivery of PRP, especially autologous activated PRP (aaPRP). Therefore, this study aimed to evaluate the safety of intravenous delivery of aaPRP.Methods: Blood was drawn from each patient and aaPRP was isolated through calcium activation and light irradiation. Each aaPRP was administered intravenously to all patients. Adverse events were documented and analyzed.Results: Six hundred eleven patients participated in this study with a total of 4244 aaPRP therapies. Quality control of autologous aaPRP showed no platelets present after both calcium activation and light irradiation. No adverse events such as allergic reaction, infection, and coagulation problems were observed on all patients over the course of the study.Conclusion: Our results showed that intravenous administration of autologous aaPRP is safe even in patients with various pathological conditions.

Highlights

Autologous platelet-rich plasma (PRP) has been a growing trend in the field of medicine due to its broad range of application and is considered safe from bloodborne diseases
Our results showed that intravenous administration of autologous activated PRP (aaPRP) is safe even in patients with various pathological conditions
Our study showed that the intravenous administration of aaPRP in patients with various pathological conditions did not cause any adverse event

Summary

Introduction

Autologous platelet-rich plasma (PRP) has been a growing trend in the field of medicine due to its broad range of application and is considered safe from bloodborne diseases. Most of the studies available have not evaluated the safety for intravenous delivery of PRP, especially autologous activated PRP (aaPRP). A total of more than 1100 proteins have been found in PRP with different functions, ranging from enzymes, growth factors, and messengers of the immune system (2). These proteins and many bioactive factors are mainly secreted by three types of granule (alpha, delta, lambda) within the platelets, with alpha granules being the most abundant. These biological processes are found to enhance wound healing and tissue regeneration (2)

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