Abstract
Objective: Evaluating the levels of pro-inflammatory cytokine (IL-6) and anti-inflammatory cytokine (IL-10) among patients with COVID-19. Methodology: A study is conducted during the period from 1st November 2021 to 1 st April 2022 , 110 patients were diagnosed as COVID-19. Their ages range between 20-81 and more years old, Who attended to AL-Amal Specialized Hospital for Communicable Diseases at first week of infection with symptoms (fever, Headache, cough, shortness of breath, diarrhea, loss of taste and smell), and compared with 50 apparently healthy individuals as control group. The diagnosis of each case was established using clinical diagnosis and confirmed by reverse transcriptase polymerase chain reaction (rt-PCR), 5 ml of fresh venous blood samples were collected from COVID-19 infected patients by sterile syringes and saved in serum separator tube (SST) with specific gel to easy sorting the serum, then left for 20 minutes at room temperature. After coagulation, sera were separated by centrifuge 4000xg for 15 minutes and directly stored at -20 ºC to be analyzed later for IL-6 and IL-10 assay.
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