Abstract
Thyme essential oil (TEO) was extracted from dried leaves of Thymus vulgaris. The air-dried aerial parts of the plant produced 1.0% yield of TEO. The detection of this essential oil’s compounds was performed by GC-MASS. The cytotoxic activity of TEO was evaluated against two human cancer cell lines, namely HeLa (human epithelial cervical cancer) and MCF-7 (human breast carcinoma). Cells grown in 96 multi-well plates were treated with six concentrations of EO (6.25, 12.5, 25, 50, 100, 200 ppm) and incubated at 37 °C for 72 hrs. Cancer cell lines elicited various degrees of sensitivity to the cytotoxic effect of essential oil. The TEO exhibited significant differences (p≤ 0.01) between the effects of all concentrations against these two human cell lines. The results showed the highest toxicity of TEO on HeLa cell line (78.67%) and MCF-7 cell line (83.60%) at 200 ppm concentration. Also the values of half-maximal inhibitory concentration (IC50) of TEO against HeLa and MCF-7 cell lines were 34.63 and 27.66 ppm, respectively. Cells treated with the IC50 of TEO showed a significant difference (p ≤ 0.01) in p53 fold expression between HeLa cell line (4.33±0.41 folds) and MCF-7 cell line (5.10±0.32 folds). In general, a dose-dependent decrease the survival of the two cell lines was observed. In addition, MCF-7 cell line revealed higher sensitivity against TEO than HeLa cell line.
Highlights
Breast cancer is considered as one of the common and well known cancers and is the main cause of death in woman throughout the world
Many studies found relations between p53 mutations and frequent genetic alterations in breast cancer, such as that observed in 30% of breast carcinomas [2] and is hardly linked with cervical cancer [3, 4]
T. vulgaris L. is a plant that is rich in essential oils and its main chemical components are the oxygenated monoterpenes and monoterpene hydrocarbons
Summary
Breast cancer is considered as one of the common and well known cancers and is the main cause of death in woman throughout the world. 28.0 min Cytotoxic effects of TEO on MCF-7 cell line Cytotoxic effects of the essential oil extracted from T. vulgaris on breast cancer (MCF-7ve) cell line were investigated using the MTT assay. The results of the MTT assay are given in Figure-2, compared withthe viability of MCF-7 breast cancer cells after 24, 48, and 72 hrs.
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