Abstract
AbstractTo address the needs of the growing aquaculture industry, we evaluated methodologies for creating gynogenetic female clones of Southern Flounder Paralichthys lethostigma. The primary objective was to determine which treatment to stop ejection of the second polar body, pressure or cold shock, would induce diploid genesis in ova that were activated with irradiated sperm and produce more viable offspring in Southern Flounder. The cold‐shock and pressure‐shock treatments with irradiated milt had similar egg‐activation rates and embryonic development rates of 1.3% (SE = 0.9) and 5.8% (SE = 2.7), respectively. However, pressure‐shock represents the most practical method for creating gynogenetic clones of the female.
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