Evaluating rumen fermentation kinetics and nutritive value of sunflower seed meal supplemented with acetonic extract of pomegranate peel using &lt;i&gt;in vitro&lt;/i&gt; gas production technique

Shabnam Nezarati,Ramin Salamatdoust-Nobar,Naser Maheri-Sis,Abolfazl Aghajanzadeh-Golshani

doi:10.4025/actascianimsci.v41i1.43281

Evaluating rumen fermentation kinetics and nutritive value of sunflower seed meal supplemented with acetonic extract of pomegranate peel using <i>in vitro</i> gas production technique

Shabnam Nezarati, Ramin Salamatdoust-Nobar + Show 2 more

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https://doi.org/10.4025/actascianimsci.v41i1.43281

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Abstract

This study was conducted to investigate effects of supplementing different levels (0.0, 0.5 and 1.0% of buffered rumen fluid) of acetonic extract of pomegranate peel on rumen fermentation kinetics of sunflower seed meal (SFM), using in vitro gas production technique. The samples were incubated in syringes containing rumen liquor obtained from three cannulated Iranian Ghezel rams for 2, 4, 6, 8, 12, 24 and 36h. Results indicated that, addition of acetonic extract of pomegranate peel resulted in increase in gas production volume in all of incubation times (p < 0.0001). Amount of gas production, also increased by increasing dose of the extract. Amounts of a (the gas production from the immediately soluble fraction), b (the gas production from the insoluble fraction) and a + b (the potential gas production) for pomegranate peel extract supplemented sunflower seed meal were higher (p < 0.05) than that of control meal. Adding pomegranate peel extract resulted in increase estimated short chain fatty acids (SCFA) production as well as digestible organic matter (DOM), metabolizable energy (ME) and net energy for lactation (NEL) content of SFM. Production of SCFA as well as DOM, ME and NEL content of SFM increased (p < 0.05) by enhancing the level of the extract supplementation. In conclusion, it can be suggest that, supplementing acetonic extract of pomegranate peel may be lead to higher ruminal fermentation and better nutritive value of SFM in ruminants.

Highlights

Banning utilization of protein sources from animal origin in livestock nutrition due to public health and safety considerations resulted in attention of animal nutritionists to using plant protein sources
Chemical compositions of SFM have been determined in the laboratory of feed analysis, Shabestar Branch, Islamic Azad University, based on standard methods (Association Official Analytical Chemist [AOAC], 2005); which dry matter (DM), crude protein (CP), ether extract (EE), Ash, neutral detergent fiber (NDF), acid detergent fiber (ADF) and non-fibrous carbohydrate (NFC) contents were 94.6, 30.0, 5.7, 5.3, 43.2, 31.2 and 15.8%, respectively (Nezarati et al, 2014)
Addition of acetonic extract of pomegranate peel led to significant increase in gas production in all incubation times

Summary

Introduction

Banning utilization of protein sources from animal origin in livestock nutrition due to public health and safety considerations resulted in attention of animal nutritionists to using plant protein sources. Sunflower seed meal (SFM) is one of the most utilized alternative oil seed meals in animal nutrition in Iran (Nezarati, Maheri-Sis, Salamatdoust-Nobar, & Aghajanzadeh-Golshani, 2014). Many researchers have emphasized on plant derivatives as rumen fermentation modifiers for improving nutritive value of feedstuffs and productivity of animals (Benchaar et al, 2008; Fugita et al, 2018; Kim et al, 2015; Ornaghi et al, 2017; Tajodini, Moghbeli, Saeedi, & Effati, 2014). Eliyahu et al (2015) reported that global production of pomegranate fruit was million metric tons per year. Estimated annual production of pomegranate processing by-products including peels, exceeds 120 thousand tons in Iran (Mirzaei-Aghsaghali et al, 2011; Nezarati & Maheri-Sis, 2016). We have previously studied nutritional value and functional efficiency of pomegranate by-products in ruminants’ nutrition

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