Evaluating production rates of particulate organic hydrogen by marine phytoplankton for estimating phytoplanktonic productivity of organically bound tritium

Abstract

To estimate the production potential of organically bound tritium (OBT) by phytoplankton from tritiated water in coastal areas adjacent to the Fukushima Daiichi Nuclear Power Plant (FDNPP), phytoplanktonic production rates of particulate organic hydrogen (POH) were evaluated in laboratory and field experiments using stable isotope tracers (2H and 13C). In the laboratory experiment, the production rate of POH was evaluated for five types of phytoplankton cultivated cultures (two diatoms, Haptophyceae, Chlorophyceae, and Cryptophyceae) at two temperatures (15 °C and 25 °C) and two 2H concentrations in the medium (1 and 5%). Additionally, the production rate of POH was especially focused on non-exchangeable POH (NE-POH) which is the chemical form of hydrogen connected tightly to organic matter. The production rates of NE-POH in the laboratory experiment varied (0.10 to 36 μmol L−1 d−1 μg-Chl a−1) with the productivity of particulate organic carbon, phytoplankton species, and temperature, with negligible influence of 2H concentrations. In the field experiment, in situ incubation of coastal seawater at water depths of 1 and 20 m with isotope tracers under light and dark conditions, respectively, was performed thrice (November 2021, May 2022, and October 2022) on the Pacific coastal ocean approximately 2 km from the land of northeast Japan. We observed variation in the production rate of POH (0.21 to 3.1 μmol L−1 d−1 μg-Chl a−1), which was theoretically explained by the data in the laboratory experiment. Using the phytoplanktonic production rate of POH obtained in this study, OBT production by phytoplankton and the subsequent accumulation potential of OBT in sediments in the coastal area adjacent to FDNPP were tentatively estimated, results of which suggested this potential to be small.