Abstract
BackgroundThyroid cancer is the most common endocrinology cancer that its incidence has increased in recent decades. miRNAs are new biomarkers in recent studies in the diagnosis and follow-up of these patients.MethodsBlood and thyroid tissue samples were obtained from two groups of included patients (PTC and benign nodules), pre- and post-operation. miRNAs were extracted from these plasma samples and were measured quantitatively. After cDNA synthesis, qPCR was carried out. Then tissue samples were investigated, and their relation to miR expression was studied. These results were analyzed by paired- and independent samples t-test, and non-parametric tests.ResultsmiR-222 and miR-181a declined in PTC patients before and after surgery, significantly (P < 0.001 for both groups), with no significant difference in control group before and after surgery (P = 0.61 for miR-222 and P = 0.06 for miR-181a). The difference between the two groups, pre-and post-operation, was statistically significant (P = 0.01 for miR-222 and P < 0.001 for miR-181a). Comparing case and control groups, pre- and post-operatively, yielded no significant difference, in miR-155-5p levels (P = 0.61 and P = 0.53, respectively). Comparing PTC and control groups before surgery showed a significant difference (P = 0.01), while no significant difference was observed comparing them after surgery, in miR146-a (P = 0.27). Our results depicted a higher miR-155-5p and miR-146a expression before surgery than after it (P < 0.001 in both groups, for both miRs). We found a significant relationship between miR-222 and BRAFV600E mutation and significantly higher levels of miR-181a with increasing tumor size in PTC patients.ConclusionmiR-222 showed overexpression in all PTC cases, which is indicative of a relation between miRNA and PTC. Also, comparing miR-181 and miR-146a showed a significant difference between cancerous and benign cases. miR-155-5p as an inflammatory factor, showed no significant changes, comparing two groups.
Highlights
Thyroid cancer is the most common endocrinology cancer that its incidence has increased in recent decades. Micro RNA (miRNA) are new biomarkers in recent studies in the diagnosis and follow-up of these patients
There was no significant difference between groups (P = 0.12)
Our study revealed a significant relationship between miR-222 and BRAFV600E mutation in papillary thyroid cancer (PTC) patients
Summary
Thyroid cancer is the most common endocrinology cancer that its incidence has increased in recent decades. miRNAs are new biomarkers in recent studies in the diagnosis and follow-up of these patients. Thyroid cancer is the most common malignancy amongst the endocrine cancers, and its incidence has increased recently, especially in case of papillary thyroid cancer (PTC), and is still rising, mostly due to increased diagnosis [1]. This growth in diagnosis might be related to diagnostic imaging utilities, as well as fine-needle aspiration (FNA) biopsy. This increase has partly led to overdiagnosis and overtreatment [2, 3]. The estimated new cases of PTC in 2017 in the United
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