Evaluating of miR-184, miR-497, miR-378, miR-103 and miR-506 expression level in non-small cell lung cancer patients tissues compared with their normal marginal tissues

Abstract

BackgroundLung cancer is one of the most common cancers worldwide whose prevalence and mortality have not any remarkable reduction in recent decades, in spite of the progression in screening and diagnostic methods. MiR-103, miR-184, miR-378, miR-497 and miR-506 are microRNAs which recent evidence has implied the possible roles of them in the carcinogenesis process. Hence, in this study we examined whether there are any significant alterations in the expression level of these micro RNAs during carcinogenesis procedure or not, and if we can use these factors as a diagnostic or prognostic biomarker for non-small cell lung cancer (NSCLC). MethodsFifty NSCLC patients participated in our study and tumor and adjacent normal tumor-free tissues were collected during the bronchoscopy. Total RNA was extracted from cells, cDNA was synthesized and then the expression level of target miRNAs was examined by quantitative Real-time PCR. Subsequently, expression levels of these genes and their correlation with clinicopathologic features of patients were analyzed with proper statistical tests. In these tests Pvalue <0.05 was considered significant. ResultsThe output data of our study showed the statistically significant down-expression of miR-103 (Fold change = 0.83, Pvalue = 0.036), miR-184a (Fold change = 0.52, Pvalue = 0.011), miR-378 (Fold change = 0.84, Pvalue = 0.041), and miR-497 (Fold change = 0.72, Pvalue = 0.021) between these micro-RNAs only mir-497 and miR-184a are potential biomarkers for NSCLC. Also, the expression levels of these miRNAs had significant relations with metastasis, lymph node involvement, and tumor cell differentiation degree and tumor size of NSCLC patients. Deregulated fifth microRNA, miR-506 was not statistically significant (Pvalue > 0.05). ConclusionAccording to the results of this study, 4 of our studied microRNAs have the potential to be used as diagnostic or prognostic biomarkers in case of confirmation of the future studies.