EVALUATING HUMAN EMBRYO CULTURE MEDIA PERFORMANCE USING C57BL-6N MOUSE EMBRYOS

Abstract

Human embryo culture media quality control is inherently challenging as it requires the use of non-human test embryos.1 Mouse embryos, most commonly of the B6D2F1 and B6C3F1 strains, are so robust that they often overestimate the success rates of in vitro fertilization. We demonstrated in a prior study that the C57BL-6N mouse embryo strain was more representative of survival probability when subtle changes were made to culture media conditions.2 Here we seek to further substantiate the utility of the C57BL-6N strain in human embryo culture media quality control by testing the strain in different culture media.