Abstract
Spectrophotometric analysis is one of the most common techniques used to quantitate nucleic acids in a solution. More specifically, the 260/280 UV absorbance ratio of the nucleic acid can be used to determine their purity [1]Huss VAR, Festl, H, Schleifer KH. Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst. Appl. Microbiol. 1983; 4(2), 184–192. . However, traditional fixed-pathlength spectrophotometers have limitations when determining the purity ratios of these molecules. In this study, the CTechTM SoloVPE® system assessed theoretical DNA purity ratios by utilizing its variable pathlength method, known as Slope Spectroscopy®. The method was evaluated by assessing the specificity, intermediate precision, repeatability, linearity, and accuracy of the theoretical purity ratios. The observed purity ratios from the SoloVPE system demonstrated great comparison to the theoretical purity ratios, verifying the SoloVPE system’s slope spectroscopy method to be preferable for this application.
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