Evaluating Desert Actinomycetes for Enzyme and Antibacterial Production

Abdulrahman A.M Alghamdi,Yaaser Q Almulaiky,Tamer S Abdelmoneim,Naif Kadasa

doi:10.22207/jpam.15.2.56

Abstract

A total of 13 Actinomycete strains were isolated from 70 soil samples collected from five locations across the Jeddah Province, while the other two locations located in Baljurashi province of Saudi Arabia. All 13 isolates were purified and subjected to enzymatic screening and antibacterial assays. The results indicated that two of these isolates (AC45 and AC69) produced both enzymes and exerted some antibacterial activity. Isolate AC45 produced more amylase and polygalacturonase (697.8 and 1498.59 units/ml, respectively) than isolate AC69; however, AC69 secreted more lipase than AC45 (6957 and 22127 unit/ml, respectively). Furthermore, both AC45 and AC69 exhibited good antibacterial activity against Staphylococcus aureus, Staphylococcus epidermidis, and Bacillus subtilis. The two isolates were identified using their 16S rRNA sequences, and the results suggest that isolate AC45 shares 99.71% similarity with Streptomyces lavenduligriseus and isolate AC69 shares 99% similarity with Streptomyces sp.

Highlights

Actinomycetes are filamentous grampositive bacteria[1], which produce hyphae[2] and are characterized by the production of conidia[3]
The results indicated that two of these isolates (AC45 and AC69) produced both enzymes and exerted some antibacterial activity
The two isolates were identified using their 16S rRNA sequences, and the results suggest that isolate AC45 shares 99.71% similarity with Streptomyces lavenduligriseus and isolate AC69 shares 99% similarity with Streptomyces sp

Summary

Introduction

Actinomycetes are filamentous grampositive bacteria[1], which produce hyphae[2] and are characterized by the production of conidia[3]. The searching for a new site, such as the deserts to take soil samples and isolate some of the Actinomycetes for the studying of secondary metabolites, which can be detected for some of the reliable materials in the field of resistance to pathogenic bacteria rather than the most common types of Streptomyces found in soil samples around the world[21 22]. We isolated a series of Actinomycete strains from the desert soil from five locations in Saudi Arabia. These isolates were evaluated for their enzyme (a-amylase, polygalacturonase, and lipase) production and antimicrobial activity against several known pathogens. Identification of the selected isolates was performed using their 16S rRNA sequences

Materials and Methods

Result

Discussion

Findings

Conflict of Interest