Abstract
The rotifer Brachionus plicatilis is one of the most important live-feed organisms used in the larval culture of marine fish. Bioencapsulation of florfenicol in rotifers was investigated by delivering it directly to the organisms as particles and the doses ranged from 50 to 1000 mg L⁻¹. Analysis of the florfenicol concentrations in rotifers was performed using high-performance liquid chromatography. The uptake increased with the dose and the enrichment time, and the highest concentration achieved in this study, 1.25 ng rotifer⁻¹, was obtained using a dose of 1000 mg L⁻¹ and 150 min of enrichment. The optimal time for satisfactory enrichment of rotifers with florfenicol was found to be dose dependent and ranged from 30 to 120 min for doses between 400 and 800 mg L⁻¹. The parameters affecting the concentrations achieved in rotifers were the size of the florfenicol particles, enrichment time and dose.
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