Abstract
Four native isolates of entomopathogenic fungi (EPF) Beauveria bassiana (B1and B2), Metarhizium anisopliae (M1 and M2 ) were isolated and evaluated against wheat aphid, Schizaphis graminum (Rondani). Three concentrations of spores suspension from each of the four fungal isolates (1×106, 1×107 and 1×108conidia) were used against adult aphid (one day old). The results showed that (B1) was the most effective according to LC50. Whereas, when testing the toxicity of crude metabolites of fungal isolates in four concentrations for each isolate, it was found that the highest effective toxin was (M1) followed by (B1), (M2) and (B2). Scanning electron microscope photographs (SEM) showed the mode of action of entomopathogenic fungi and its ability to colonize and how it infects aphid.
Highlights
Cereal aphids are the serious pests attacking cereal crops, wheat which caused yield losses either directly by sucking the sap of the plants or indirectly by transmitting viral and fungal diseases (Abro et al, 2004)
The present work was carried out as an attempt to investigate and suggest some alternative agents be incorporated into IPM control of wheat aphid; S. graminum by determining the toxicity of four isolates of entomopathogenic fungi Beauveria bassiana (Blas.) and Metarhizium anisopliae (Metsch.) and their metabolites
S. graminum adult females will be treated under laboratory conditions, with entomopathogenic fungi and their extracts to evaluate their effects and efficiency as natural insecticides
Summary
Evaluate the Effects of Entomopathogenic Fungi Isolates on Wheat Aphid, Schizaphis graminum (Rondani) (Hemiptera: Aphididae). 1-Plant Protection Research Institute, Agricultural Research Center, Dokki, Giza, Egypt 2-Al-Azhar University, Faculty of Science (Girls Branch) Naser City, Cairo, Egypt
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