Abstract
<p><strong>ABSTRACT. </strong><em><strong>Background:</strong></em> The root canal filling technique named Hybrid-Mixed Condensation, combines the advantages of cold lateral and warm vertical condensation. The ability of avoiding microbial microleakage has not been proven. <em><strong>Purpose:</strong></em> To evaluate the differences of microbial microleakage using <em>Enterococcus faecalis</em>, in canals obturated with five different techniques: lateral, warm vertical, WaveOne® single cone, Guttacore®, and Hybrid-mixed condensation. <em><strong>Methods:</strong></em> 50 single-rooted human premolars extracted for orthodontic reasons were biomechanical prepared with primary file of WaveOne® system. Teeth were divided into 5 groups using different obturation techniques: single cone with WaveOne® Primary, lateral condensation using 2 % gutta-percha cones, Guttacore® 30, warm vertical condensation using down packing in a WaveOne® Primary cone and backfill with alpha gutapercha of Beefill®, and the hybrid mixed condensation modifying the lateral condensation with heat and a backfill using Beefill®. <em>Enterococcus faecalis</em> was inoculated in the coronal third and apices were immersed in brain heart infusion broth with phenol red incubated at 37 °C for 12 weeks. Microfiltration was determined with color change and turbidity of the medium. Specimens were observed by scanning electron microscopy. <em><strong>Results:</strong></em> Only 11 teeth (22 %) were positive for leakage. 46 % with single cone, 30 % with Guttacore®, 20 % with lateral condensation, 10 % with warm vertical condensation and no microleakage was found for Hybrid-Mixed Technique over the period of 12 weeks of study. <em><strong>Conclusion:</strong></em> Hybrid Mixed Technique showed to be the most efficient technique to get three-dimensional seal and prevent microbial contamination of canals in the endodontic therapy.</p>
Highlights
The root canal filling technique named Hybrid-Mixed Condensation, combines the advantages of cold lateral and warm vertical condensation
Only teeth (22%) were positive for leakage. 46% with single cone, 30% with Guttacore®, 20% with lateral condensation, 10% with warm vertical condensation and no microleakage was found for Hybrid-Mixed Technique over the period of weeks of study
Results: Only 11 teeth (22%) were positive for leakage. 46% with single cone, 30% with Guttacore®, 20% with lateral condensation, 10% with warm vertical condensation and no microleakage was found for Hybrid-Mixed Technique over the period of 12 weeks of study
Summary
Con el aval del Comité de Investigación y Ética de la Facultad de Odontología de la Pontificia Universidad Javeriana, se realizó un estudio experimental in vitro para el cual se tomaron por conveniencia 50 dientes premolares de humanos, unirradiculares, extraídos por indicación ortodóntica, donados por pacientes de consultorios odontológicos privados en las ciudades de Alajuela y San José (Costa Rica). Se verificó la permeabilidad del conducto con una lima tipo K # 10 (Dentsply Maillefer®, Suiza), determinando visualmente la longitud de trabajo a −0,5 mm del foramen mayor. Grupo 1 (CU): se utilizó cono no estandarizado Primary de WaveOne® hasta la longitud de trabajo, el cual se cortó en coronal y se compactó verticalmente en el tercio cervical con un condensador de acero inoxidable B50 (Dentsply Maillefer®, Suiza). Grupo 2 (CL): se utilizó un cono estandarizado calibre 35 del 2 % de conicidad (Dentsply Maillefer®, Suiza), espaciador A30 (Dentsply Maillefer®, Suiza) y conos accesorios estandarizados # 15 y 20 del 2 % de conicidad (Dentsply Maillefer®, Suiza), que cortaron la porción excedente de los conos de gutapercha 2 mm por debajo de la línea amelocementaria y se compactó verticalmente con un condensador de acero inoxidable B50 (Dentsply Maillefer®, Suiza).
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