Evaluación del recolector de orina en la toma de muestra de líquido seminal y tinción diferencial de fluorescencia en espermatozoides

Effect of injected spermatozoa morphology on the outcome of intracytoplasmic sperm injection in humans

Study of human, knockout mice, and in-vitro models revealed CEP78 absence is the genetical cause of cone-rod dystrophy and male infertility with multiple morphological abnormalities of sperm flagella, CEP78 interacted with IFT20 and TTC21A to modulate cilliogenesis and centriole length.

Study of human, knockout mice, and in-vitro models revealed CEP78 absence is the genetical cause of cone-rod dystrophy and male infertility with multiple morphological abnormalities of sperm flagella, CEP78 interacted with IFT20 and TTC21A to modulate cilliogenesis and centriole length.

Semen analysis by electron and fluorescence microscopy in a case of partial hydatidiform mole reveals a high incidence of abnormal morphology, diploidy, and tetraploidy

Abnormal sperm count and motility on semen analysis are not sufficiently predictive of abnormal Kruger morphology

Sperm Ion Channels: Molecular Targets for the Next Generation of Contraceptive Medicines?

In vitro effects of coital lubricants and synthetic and natural oils on sperm motility

Volume, motility, concentration and sperm abnormalities represent important parameters within ram semen production examination from the viewpoint of quantitative and qualitative meaning. Without the sperm concentration analysis and without the establishment of live and viable sperm number there can’t be conceived a rational dilution so as to obtain a suitable number of insemination doses from each ejaculate and from each ram, in this way to realize a selection pressure through the agency of the sires (Fitzgerald and Stellflug, 1998). The crossing between Tzigai and Suffolk breeds is profitable thanks to high meat production and some superior quantitative and qualitative semen parameters that come the last one. The aim of the present study was to compare some semen parameters at these two breeds. Immediately after semen collection, there were exams a number of 8 semen samples from Tzigai rams and 16 samples from Suffolk rams, analyzing the sample volume, the sperm motility and concentration, the number of sperm/sample inclusive the sperm abnormalities. The differences between treatments were analyzed by ORIGIN7 and interpreted using the Student test. The medium value of the samples volume was by 0.43±0.01 ml at Tzigai rams and respectively 0.48±0.02 ml at Suffolk rams, of the sperm motility was by 0.93±0.01 decimal notes and respectively by 0.94±0.01, of sperm concentration was by 1.73±0.04 billions sperm/ml and respectively by 2.05±0.04 billions sperm/ml, of sperm number per sample was by 0.75±0.01 sperm/sample and respectively by 0.99±0.03 sperm/sample, of sperm head abnormalities was by 4.62±0.18% and respectively by 4.00±0.20%, of sperm intermediary piece abnormalities was by 5.25±0.31% and respectively by 4.31±0.11%, of sperm tail abnormalities was by 4.50±0.18% and respectively by 3.68±0.11% and of total sperm abnormalities was by 14.37±0.41% and respectively by 12.00±0.20%. Therefore were obtained non significant differences respect the samples volumes (t=1.58), the sperm motility (t=1.07) and the sperm head abnormalities (t=1.96), distinctly significant differences respect the sperm intermediary piece abnormalities (t=3.39) and the sperm tail abnormalities (t=3.77) and very significant differences respect the sperm concentrations (t=4.89), the sperm number per sample (t=4.67) and the total abnormalities (t=5.77). Results a superiority of Suffolk rams semen against the Tzigai rams semen respect the qualitative semen parameters.

Study question Is the supplementation of the cryosolution with coconut water (CW) improve the motility and DNA integrity of spermatozoa after cryopreservation? Summary answer Coconut water as an additive to the cryosolution found to decrease the sperm DNA fragmentation and improve the sperm motility post-thawing. What is known already Despite the benefits of cryopreservation, it has adverse effects on sperm cell in different scenarios. Decrease in motility, viability and DNA fragmentation are the most common effects of cryopreservation on sperm function. The freezing/thawing shock produces physical and chemical stresses on the spermatozoa that could change the composition of the lipids in their plasma membrane resulting in increased production of reactive oxygen species (ROS) and increases the oxidative stress. Thus, supplementing the cryosolution with antioxidants may improve the recovery rate of cryopreserved spermatozoa by reducing the oxidative damage. Coconut water is characterized by its high contents of proven antioxidant properties. Study design, size, duration The study was conducted on 70 semen samples from patients attended the fertility center in Al-Sadder medical city, Najaf, Iraq, for routine semen analysis over a period of ten months from March to December 2022. All the patients gave their consent for participation in this study. The samples had normal semen parameters according to World Health Organization (WHO) 2010 standard criteria. Participants/materials, setting, methods Sperm motility and DNA fragmentation index (SDF) test were done for each sample, then, each semen sample divided to three parts: P1: cryopreserved with glycerol-containing cryosolution only (SpermFreezTM), P2: cryopreserved with glycerol-containing cryosolution and 5%coconut water (CW), and P3: cryopreserved with glycerol-containing cryosolution and 10% CW. After 1-month vapor-dependent cryopreservation, all samples were thawed and the sperm motility assed using computer-assisted sperm analyzer (CASA) and DFI was evaluated using Acridine orang stain method. Main results and the role of chance The percentage of total sperm motility and progressive motility in fresh samples were (39% and 35% respectively) and the level of sperm DNA fragmentation was (21%). The total and progressive sperm motility decreased significantly (P < 0.05) post-thawing, while the SDF increased. After cryopreservation the percentage of total and progressive sperm motility exhibited a significantly elevated levels in P2 (10.1% and 7.9% respectively) and P3 (14.6% and 11.3% respectively) than in P1 (6.8% and 4.2% respectively), however, the level of DFI significantly (P < 0.05) decreased in both P2 (27.4%) and in P3 (24.5%) than in P1 (30.1%). In comparison between the two concentrations of CW, P3 recorded a significant increase in the recovery of sperm total and progressive motility (14.6% and 11.3% respectively) than P2 (10.1% and 7.9% respectively). Furthermore, the level of DFI in P3 (24.5%) decreased significantly than its level in P2 (27.4%). Limitations, reasons for caution This study was to evaluate the efficiency of CW in the improvement of human sperm motility and DNA integrity post-thawing. Further studies required to evaluate the effect of using CW in sperm cryopreservation on the success rate of the conventional in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI). Wider implications of the findings In this study, we found that the additive of CW to the glycerol-containing cryosolution (SpermFreezTM) can improve the human sperm motility and DNA integrity post-cryopreservation. The best recovery rate of sperm motility and DNA integrity was with the supplementation of 10% of CW to the cryosolution. Trial registration number Not applicable

Functional and molecular characterization of voltage gated sodium channel Nav 1.8 in bull spermatozoa

Effect of age on spermiogram of Holstein Friesian × Sahiwal crossbred bulls

Objective To investigate the effects of L-carnitine in different concentrations on DNA fragmentation index (DFI), mitochondrial membrane potential (MMP) and acrosome reaction (AR) during human sperm cryopreservation. Methods Forty patients were randomly selected from the June 2017 to November 2017 in the Second Affiliated Hospital of Zhengzhou University. Experiments were carried out on the patient consent. In addition, the semen samples were collected by masturbation method.All semen samples were fully mixed and divided into five groups: group A with fresh semen as control, group B0 with conventional cryoprotectant, group B5 with 5 mmol/L L-carnitine, group B10 with 10 mmol/L L-carnitine, group B20 with 20 mmol/L L-carnitine. The semen samples were frozen by liquid nitrogen vapor, and then were thawed 48h later. Computer-aided sperm analysis was used to detect the progressive motility (PR) and viability of spermatozoa before and after cryopreservation. Flow cytometry was used to detect DFI, MMP and AR in each group. Results The PR, motility and MMP after freezing were significantly lower than those before freezing. The sperm DFI was higher than that before freezing.There was no difference in the likelihoods of the PR on motility between four frozen groups (P>0.05). The sperm DFI of spermatozoa were as follows: B10 0.05). Conclusions Sperm cryopreservation would lead to frozen injury, which can reduce PR, sperm motility, MMP Besides, sperm cryopreservation also lead to sperm DFI increasing; cryoprotectant added with 10 mmol/L L-carnitine or 20 mmol/L L-carnitine group can reduce the sperm DFI and alleviate decrease in MMP. In this way, the effects of sperm cryopreservation can be improved. Moreover, 10 mmol/L of L-carnitine can be used as an active ingredient to protect spermatozoa in human sperm cryopreservation. Key words: L-carnitine; Sperm cryopreservation; DNA fragmentation; Mitochondrial membrane potential; Acrosome reaction

Density gradient centrifugation (DGC) and swim-up techniques have been reported for semen preparation in assisted reproductive techniques in humans. We investigated whether semen preparation using a combination of DGC and swim-up techniques could effectively decrease morphologically abnormal human sperms at the ultrastructural level. Semen samples were obtained from 16 infertile males and fractionated by swim-up following DGC. Ultrastructural abnormalities of sperms obtained from original semen, lower layer of swim-up following DGC, and upper layer of swim-up following DGC were analyzed by transmission electron microscopy. The correlation among ultrastructural head abnormality in sperms from the upper layer of swim-up, fertilization in in vitro fertilization, and pregnancy after embryo transfer was also investigated. Furthermore, sperms with DNA fragmentation in the samples processed via a combination of DGC and swim-up was assessed in a sperm chromatin structure assay. Ultrastructural abnormalities in sperm heads and tails in the upper layer after swim-up following DGC was the lowest among the three groups. Sperms with nuclear vacuoles were the most difficult to eliminate using a combination of DGC and swim-up in all types of head abnormalities. A negative correlation was confirmed between the fertilization rates of intracytoplasmic sperm injection and head abnormality of sperms obtained from the upper layer of the swim-up following DGC. Sperms with DNA fragmentation were effectively decreased using the combination of two techniques. In conclusion, the combination of DGC and swim-up effectively decreased the number of sperms with ultrastructural abnormalities both in the head and in the tail. However, sperms with ultrastructural abnormalities that cannot be completely decreased using a combination of DGC and swim-up may impair fertilization in some cases of intracytoplasmic sperm injection.

Preservation of mithun ( Bos frontalis) semen at refrigeration temperature

With the motile sperm organelle morphology examination (MSOME), spermatozoa morphology may be assessed directly on motile spermatozoa at high magnification (up to 6600×). This procedure describes more precisely spermatozoa abnormalities, especially head vacuoles. However, no consensus has been established concerning normal or abnormal MSOME criteria. The aim of our study was to define MSOME vacuole criteria assessed objectively with a digital imaging system software to establish a potential relationship between conventional semen parameters. A total of 440 semen samples were obtained from males consulting in Rouen University Hospital Reproductive Biology Laboratory. Conventional semen analysis (volume, sperm concentration, progressive motility, vitality and morphology) and MSOME assessment {sperm head length, width and area as well as vacuole number, vacuole area and relative vacuole area to sperm head [RVA (%) = [vacuole area (μm(2))/head area (μm(2))] × 100)]} were performed for each semen sample. Among our 440 males, 109 presented normal conventional semen parameters and 331 abnormal ones. Sperm head vacuoles were significantly larger in abnormal semen samples (p < 0.0001). RVA was the most discriminative MSOME criterion between normal and abnormal semen samples according to ROC curves analysis, and was negatively correlated with poor sperm morphology (r = -0.53, p < 0.0001). We concluded to (i) the normal occurrence of vacuoles in sperm head whatever the normality or abnormality of semen parameters, (ii) the discriminative function of the RVA to distinguish semen samples with normal and abnormal parameters, and (iii) the strong correlation between high RVA and poor sperm morphology.