Abstract
BackgroundEnterovirus 71 (EV71) is the most common causative pathogens of hand, foot and mouth disease (HFMD) associated with severe neurological complications. There is a great need to develop prophylactic vaccine against EV71 infection.ResultsEV71 virus-like particle (VLP) was produced in yeast expression system by the co-expression of four EV71 structural proteins VP1–VP4. Immunization with the recombinant VLPs elicited potent anti-EV71 antibody responses in adult mice and anti-VLP sera were able to neutralize EV71 virus in vitro. Neonatal mice model demonstrated VLP immunization conferred protection to suckling mice against the lethal viral challenge.ConclusionsCo-expression of four EV71 structural proteins VP1–VP4 in yeast expression systems is an effective method to produce EV71 VLPs. VLP-based vaccine shows great potential to prevent EV71 infection.
Highlights
Enterovirus 71 (EV71) is the most common causative pathogens of hand, foot and mouth disease (HFMD) associated with severe neurological complications
Generation and characterization of EV71 virus-like particle (VLP) EV71 capsid is composed of 60 copies of each of the four viral structural proteins VP1, VP2, VP3 and VP4
Our data indicate that co-expression of four viral structural proteins VP1, VP2, VP3 and VP4 in yeast cells can definitely lead to the formation of EV71 VLPs
Summary
Enterovirus 71 (EV71) is the most common causative pathogens of hand, foot and mouth disease (HFMD) associated with severe neurological complications. The virion is around 25–30 nm in diameter containing a single-stranded RNA viral genome of approximately 7500 nucleotides [1,2,3]. The viral genome is packaged in an icosahedral capsid which is composed of 60 copies of structural proteins. Highresolution structural analysis showed that VP1-3 form a EV71 has been identified as one of the major etiological agents of hand, foot and mouth disease (HFMD) [8, 9]. A number of HFMD epidemics caused by EV71 infection occurred in the Asia–Pacific region and are associated with severe neurological complications such as aseptic meningitis, poliomyelitis-like paralysis and brainstem encephalitis. No appropriate vaccine is yet available to prevent EV71 infection
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