Abstract
A working group was established in the frame of EUPHRESCO Phytosanitary ERA-NET Project aimed to compare and validate diagnostic protocols for the detection of “flavescence doree” phytoplasma in grapevine. Seven molecular protocols were compared in interlaboratory trials starting from extracted DNAs prepared in one laboratory. The tested molecular protocols consisted of universal and group-specific real-time and conventional nested PCR assays. Real-time PCR protocols, generally, revealed higher values of diagnostic sensitivity and diagnostic specificity than conventional PCR protocols. These preliminary results require a statistical analysis to confirm the performances of the protocols.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.