Eugenol-Functionalized Magnetite Nanoparticles Modulate Virulence and Persistence in Pseudomonas aeruginosa Clinical Strains.

Hamzah Basil Mohammed,Lia-Mara Ditu,Grigore Mihaescu,Alina-Maria Holban,Mariana Carmen Chifiriuc,Sajjad Mohsin I Rayyif,Alexandru Mihai Grumezescu,Ovidiu-Cristian Oprea,Alexandra Catalina Birca,Irina Gheorghe,Carmen Curutiu

doi:10.3390/molecules26082189

Hamzah Basil Mohammed, Lia-Mara Ditu + Show 9 more

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https://doi.org/10.3390/molecules26082189

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Abstract

Efficient antibiotics to cure Pseudomonas aeruginosa persistent infections are currently insufficient and alternative options are needed. A promising lead is to design therapeutics able to modulate key phenotypes in microbial virulence and thus control the progression of the infectious process without selecting resistant mutants. In this study, we developed a nanostructured system based on Fe3O4 nanoparticles (NPs) and eugenol, a natural plant-compound which has been previously shown to interfere with microbial virulence when utilized in subinhibitory concentrations. The obtained functional NPs are crystalline, with a spherical shape and 10–15 nm in size. The subinhibitory concentrations (MIC 1/2) of the eugenol embedded magnetite NPs (Fe3O4@EUG) modulate key virulence phenotypes, such as attachment, biofilm formation, persister selection by ciprofloxacin, and the production of soluble enzymes. To our knowledge, this is the first report on the ability of functional magnetite NPs to modulate P. aeruginosa virulence and phenotypic resistance; our data highlights the potential of these bioactive nanostructures to be used as anti-pathogenic agents.

Highlights

IntroductionThe subinhibitory concentrations (MIC 1/2) of the eugenol embedded magnetite NPs (Fe3 O4 @EUG) modulate key virulence phenotypes, such as attachment, biofilm formation, persister selection by ciprofloxacin, and the production of soluble enzymes
Microbiology & Immunology Department, Faculty of Biology, University of Bucharest, Research Institute of the University of Bucharest—ICUB, University of Bucharest, 050657 Bucharest, Romania; Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Materials Science, Politehnica University of Bucharest, 011061 Bucharest, Romania; Citation: Mohammed, H.B.; Rayyif, Md
By comparing already published magnetite X-ray spectra [23,24] with the patterns resulting from the X-ray diffraction analysis of eugenol functionalized magnetite samples, it could be observed that the presence of the antimicrobial compound eugenol did not affect the magnetite-specific crystalline structure

Summary

Introduction

The subinhibitory concentrations (MIC 1/2) of the eugenol embedded magnetite NPs (Fe3 O4 @EUG) modulate key virulence phenotypes, such as attachment, biofilm formation, persister selection by ciprofloxacin, and the production of soluble enzymes. To our knowledge, this is the first report on the ability of functional magnetite NPs to modulate P. aeruginosa virulence and phenotypic resistance; our data highlights the potential of these bioactive nanostructures to be used as anti-pathogenic agents. Natural products represent a great source of diverse and useful chemical compounds, which have the potential to be used in many biomedical applications, including the control of severe infections [7] They represent a natural and ecological alternative with great potential in the design of published maps and institutional affiliations

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