Abstract

Vegetative propagation of tulips in vitro, not performed until now, is obtained using as explants scales of daughter bulbs collected in winter. Twenty-five percent of these explants give rise to neoformed buds within 6 months. These buds evolve into bulbs which are able to develop in the soil. Their ontogeny is studied histologically, from the beginning of the culture to the neoformed bulb formation. A treatment with low temperature and darkness at the beginning of the culture period promotes an earlier bud initiation.

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