Abstract

The rate of germination of spores of B. stearothermophilus has been examined under anaerobic conditions in the presence of germinating agents and food extracts. L-alanine (10mM/l) at pH 6 to 7 was an effective agent and produced over 99% germination in 30min. Water extracts of green peas, green beans and carrots and the brine of these canned products were all effective in stimulating germination and this effect appeared to be related to the presence of free alanine. The total number of viable cells (germinated and dormant) in the germination medium or remained constant or decreased for several hours after the beginning of germination.

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