Etiology and Antimicroba Susceptibility of Udder Pathogens from Cases of Subclinical Mastitis in Dairy Ettawa Crosbread Goat (PE) in Kulonprogo Yogyakarta

Abstract

Subclinical mastitis in Ettawa crosbreeds (PE) is an inflammatory disease that no clinical symptoms, but there is an increase the number of somatic cells and causes decrease milk production which economically detrimental. The aim of these study was to isolation of bacteria that causing subclinical mastitis in PE goats and their susceptibility with antimicrobial. A total of 37 PE goats from 5 farms in Kulonprogo were tested by California Mastitis Test (CMT). PE goats were said subclinical mastitis if the CMT test positive (++) or (++). Isolation and identification bacteria from PE goat subclinical mastitis by bacteriological analytical manual. Coagulase test with tube coagulation and antimicroba susceptibility with agar diffusion. Subclinical mastitis in PE goats at Kulonprogo farm is 10/37 (27%) which caused by S. intermedius positive coagulase 4/4 (100%), S. aureus negative coagulase 7/10 (70%), S. aureus positive coagulase 3/10 (30%), and E. coli 1/10 (10%). S. intermedius positive coagulase was resistant to ampicillin, tetracycline, and sulfamethoxazole 2/4 (50%) respectively. S. aureus negative coagulase was resistant to ampicillin 2/7 (28.6%), penicillin 1/7 (14.3%), and sulfamethoxazole 1/7 (14.3%). S. aureus positive coagulase was resistant to ampicillin 1/3 (33.3%), penicillin 3/3 (100%), sulfametoxazole 2/3 (66.7%), and tetracycline group 1/3 (33.3%). Subclinical mastitis PE goats in Kulonprogo was caused by S. intermedius positive coagulase and S. aureus negative coagulase which resistant to penicillin and sulfamethoxazole.