Etidronic acid adduct and bis (2-pyridyl-1,2,4-triazolyl-3) butane: properties and effect on pain sensitivity

Abstract

The article presents the results of experimental studies on the effect of etidrononic acid adduct and bis(2-pyridyl-1,2,4-triazolyl-3)butane (EA+BTB) on the pain sensitivity of male and female rats. This substance is created on the precursors basis, which have a pronounced analgesic activity in acute pain when injected once into male and female rats. Experiments were performed on 30 male and 30 female adult Wistar rats weighing 200-220 gr. Pain sensitivity of animals was assessed 1 hour after a single intraperitoneal injection of the studied solution substances by acute pain tests (“tail-flick” test, Randall-Sellitto test, “hot plate” test). It was found out that EA+BTB, when injected once in doses of 5 and 50 mg/kg, significantly changes the pain sensitivity of male and female rats in acute pain tests, showing gender specificity of the analgesic effect with the participation of various pain mechanisms (spinal and supraspinal) and nociception components (perceptual and mechanical). The compound EA+BTB is recommended for further in-depth preclinical tests of its analgesic activity, decoding its physiological and molecular mechanisms in order to create an improved prototype of a new generation drug based on EA+BTB.