Abstract
The cDNA encoding a putative ethylene response sensor (ERS) type ethylene receptor OgERS1 of Oncidium was cloned and the deduced amino acid sequence of OgERS1 was structurally characterized. Phylogenetic analysis of amino acid sequences of ethylene receptor homologues indicated that OgERS1 was closely related to other members of ethylene receptors from monocotyledonous plants. OgERS1 was expressed abundantly in roots and flower buds, and a lesser extent in pseudobulbs, leaves, and fully opened flowers. OgERS1 mRNA levels in fully opened flowers during the natural senescence increased with days after cutting and reached the maximum in the fifth day of bloom. The accumulation of OgERS1 mRNA began to decrease at the day when ethylene raised abruptly and the flowers started to wilt. The expression of OgERS1 mRNA in pollinia de-capped Oncidium flowers showed an increasing and then decreasing trend toward the late stage of flower senescence as in un-dislodged fully opened flowers, and the overall expression level was decreased throughout the senescence period referred to un-dislodged flowers. Our results concluded that the expression of ethylene receptor OgERS1 is differentially expressed in different organs and the pollinia cap dislodgment alleviated the mRNA levels of ethylene receptor initiated by exogenous ethylene treatments.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.