Abstract
Nonflowering-sized Dutch iris bulbs 'Telstar' were treated at 20 °C in a flow-through system with ethylene-free air or air containing 10 μL.L-1 of ethylene (C2H4) for 1 or 24 hours. Following the treatment, bulbs were placed in a 10 °C cooler for 5 weeks before planting and subsequently forced in a 20 °C day/18 °C night glasshouse. Results demonstrated that a 24-hour C2H4 treatment induced earlier flowering as well as percent flowering. Flowering responses of bulbs treated with C2H4 for 1 hour were the same as those treated with air. The 24-hour ethylene treatment did not affect the combined fresh weight of the daughter bulb and bulblets but reduced the number of bulblets produced by each. The effects of ethylene on the protein changes in the apex were examined at two time periods: immediately after the treatment and after the cold treatment. Each sample consisted of proteins extracted from three apices. There are significant changes in one-dimensional SDS PAGE protein patterns of bulbs apices after treatment with ethylene. However, the differences are apparent after only 1 hour of ethylene treatment. The cold treatment resulted in minimal further changes in protein patterns.
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