Ethylene and in vitro rooting of hazelnut (Corylus avellana) cotyledons

Abstract

Ethylene may be one of the many factors that play a role in rooting. However, in some studies ethylene promoted rooting, while in others it was inhibitory or had no effect. Using cotyledons of hazelnut (Corylus avellana L. cv. Casina) observations were made of the effect of ethylene precursors on adventitious root formation. l‐methionine (Met) or 1‐aminocyclopropane‐1‐carboxylic acid (ACC) added to a standard indole‐3‐butyric acid (IBA)‐kinetin‐containing medium did not enhance rooting, while 2‐chloroethylphosphonic acid (CEPA) did. The ethylene inhibitor, aminoethoxyvinylglycine (AVG), inhibited root formation, but its effect was reversed by ACC when cotyledonary segments were transferred to rhizogenic medium plus ACC at day 10. Ethylene production by cotyledons cultured on rhizogenic medium or rhizogenic medium plus CEPA was high at the beginning of rooting. Thus, the wound‐induced ethylene is a key stimulatory factor in the formation of root primordia. The data support the hypothesis that ethylene plays a positive role in root formation.