Ethylacetate extract of Aquilaria crassna preserve actin cytoskeleton on simulated ischemia induced cardiac cell death

Abstract

This paper investigates the effect of ethylacetate extract of Aquilaria crassna Pierre ex Lecomte on simulated ischemia-induced cytoskeleton disorganization in cardiac myoblast cell line, so as to provide some evidence for its traditional use. Rat cardiac myoblast cell line (H9c2), were subjected to 2 h simulated ischemia and 24 h of reperfusion, in the presence and absence of the ethylacetate extract of A. crassna. The cellular viability was determined by the reduction of 3-(4,5-dimethyl-2-thiazol)-2,5-diphenyl-2H-tetrazolium bromide (MTT) by mitochondrial reductases. Cells were pre-treated with 5 mg/ml of A. crassna extract for 1 h before, or at the beginning of 40 min simulated ischemia. The F-actin cytoskeleton organization was assessed in cells treated with 5 mg/ml prior to simulated ischemia. Cells were fixed and stained for F-actin by phalloidin-conjugatedtetramethylrhodamine isothiocyanate (TRITC) and observed under fluorescence microscopy. The results showed that 2 h of simulated ischemia significantly produced cellular injury and cell death, which was significantly inhibited when treated with 5 mg/ml of the ethylacetate extract of A. crassna (p < 0.05). Treatment of the ethylacetate extract of A. crassna significantly preserved actin cytoskeleton organization. In conclusion, the ethylacetate extract of A. crassna reduced ischemia induced cell death preserve cytoskeleton organization. Key words: Aquilaria crassna, ischemic heart disease, myocardial ischemia, actin cytoskeleton.